Human papillomavirus genotype attribution in premalignant-malign cervical lesions: a retrospective tertiary center results

ABSTRACT

Aim: Oncogenic Human papillomavirus (HPV) types play an important role in the development of cervical cancer. The cervix is an easily accessible organ that significantly improves the prognosis with early diagnosis by screening tests, and subsequent early treatment planning. In our study, we wanted to investigate the importance and prevalence of HPV subtypes in cervical neoplastic lesions.

Materials and Methods: 296 women who applied to the gynecological oncology clinic in our hospital and underwent colposcopic biopsy based on HPV tests were evaluated retrospectively. Colposcopic biopsy samples were evaluated by histopathological examination and HPV samples were evaluated by polymerase chain reaction (PCR) and DNA typing was performed.

Results: Among the HPV types in our patients, the most common HPV type 16 was detected. Among the high-risk HPV types, the 5 most common types are 16,31,18,51, and 52, respectively. While the most common HPV types were 16,35,31 in high-grade squamous intraepithelial lesions (H-SIL), and 16,51,31 in low-grade squamous intraepithelial lesions (L-SIL). In our region, a cervical premalignant or malignant lesion was detected in 129 of 296 patients who had a colposcopic cervical biopsy. In 110 of these 129 patients, HPV was detected in etiology, and HPV was not detected in 19/129 of them.

Conclusion: The HPV virus is the most important risk factor in the etiology of cervical cancer. The content of vaccines planned to be developed against HPV is the most common and high-risk group subtypes and will be promising in preventing cervical cancer.

Key words: HPV subtypes, cervical intraepithelial neoplasia, cervix carcinoma